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KinaseSTAR Akt Activity Assay Kit
Product Overview
Kit Summary:
• Detection method- Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions
• Sample type- Cell and Tissue lysates
• Species reactivity- Mammalian
• Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate.
Features & Benefits:
• Simple procedure
• The kit is suitable for both research and drug discovery
• The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Kit components:
• Kinase Extraction Buffer
• Akt Specific Antibody
• Protein A Sepharose
• GSK-3α Protein/ATP Mixture
• Kinase Assay Buffer
• Phospho-GSK-3α Specific Antibody
Description:
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Phosphorylation of the GSK-3α can be analyzed by Western blot analysis using the phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |