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ApoBrdU-IHC DNA Fragmentation Assay Kit
Product Overview
Kit Summary:
• Sample type- Tissue sections and fixed cell preparations
• Species reactivity- Mammalian
• Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
Features & Benefits:
• Simple procedure; convenient
• The assay is sensitive and stable
• The assay offers an one-step labeling of apoptotic cells.
Kit components:
• Control Slides-pos/neg
• Blocking Buffer
• H₂O₂/Urea Tablets
• Protease K
• DAB Tablets
• TdT Enzymes
• Br-dUTP
• 200X Conjugate
• 5X Reaction Buffer
• Anti-BrdU-Biotin mAb
• Methyl Green
Description:
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |