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CaspSCREEN Caspase Screening Kit
Product Overview
Kit Summary:
• Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).
• Sample type- Live cells
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25 and 100 assays)
• Applications- Detection of activated caspases in living cells.
Features & Benefits:
• Simple one-step procedure; takes only 30 minutes
• Fast and convenient
• D₂R is more cell-permeable than other fluorometric caspase substrates
Kit components:
• D₂R Reagent
• DTT (1 M)
• D₂R Incubation Buffer
Description:
Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |