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Leptin Antibody
Product Overview
Antibody Target: Leptin
Alternative Name: LEP; OB; OBS; Obese Protein, Leptin
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E. coli-expressed recombinant human leptin
Accession#: AAH69323
Gene ID: 3952
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-human Leptin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human
Application: Western blot
Application & Usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Leptin (Cat.# 4366-1) can be used as a positive control.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acide residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |