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MCP-1 Antibody
Product Overview
Antibody Target: MCP-1/MCAF
Alternative Name: MCP1, MCP-1, MCP 1, monocyte chemoattracctant protein-1, monocyte chemoattracctant protein 1, monocyte chemoattracctant protein
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: E. coli-expressed rat MCP-1 (a.a. 1-73)
Accession#: P14844
Gene ID: 24770
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-rat MCP-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
Purification: Affinity purified
Species Reactivity: mouse, rat
Application: Western blot, Neutralization
Application & Usage: Western blot analysis (0.5-2 µg/ml). From researcher’s feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |