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IFN-gamma, murine recombinant
A critical cytokine for innate and adaptive immunity
Product Overview
Alternate Names / Synonyms: Murine IFN-γ, IFN-γ, IFNγ, IFN γ, m- IFN-γ, rm- IFN-γ, recombinant murine IFN-γ, recombinant IFN-γ, IFN
Gene Symbol: IFNG
Accession #: P01580
Gene ID: 15978
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 10 mM Na2PO4, pH 7.0 + 25 mM NaCl
Molecular Weight: 15.6 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁷ units/mg.
Reconstitution Instructions: Reconstitute in ddH₂O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Recombinant murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |