Alternate Names / Synonyms: human G-CSF, G-CSF, GCSF, G CSF, h-G-CSF, rh-G-CSF, recombinant human G-CSF, recombinant G-CSF Gene Symbol: CSF3 Accession #: P09919 Gene ID: 1440 Source: E. coli Appearance: Lyophilized protein Physical Form Description: Lyophilized from 5 mM Sodium Citrate, pH 4.0. Molecular Weight: 19.7 kDa Purity by SDS-PAGE: ≥98% Purity by HPLC: ≥98% Endotoxin Level: <0.1 ng/μg Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁸ units/mg. Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. Storage Temp.: -20°C Shipping: Gel pack Background Information: Granulocyte Colony stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues. Handling: Centrifuge the vial prior to opening. |