Murine CD40Ligand/TRAP

CD40Ligand/TRAP, murine recombinant
A protein primarily expressed on activated T cells and involved in immune functions
Product Overview
Alternate Names / Synonyms: Murine CD40Ligand/TRAP, CD40Ligand/TRAP, m-CD40Ligand/TRAP, rm-CD40Ligand/TRAP, recombinant murine CD40Ligand/TRAP, CD40 Ligand, TRAP, recombinant CD40 Ligand/ TRAP
Gene Symbol: CD40-L
Accession #: P27548
Gene ID: 21947
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine.
Molecular Weight: 16.4 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: Determined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED₅₀ for this effect is <0.1 µg/ml.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.