|
Cytochrome c Antibody (Clone 7H8.2C12)
Product Overview
Antibody Target: Cytochrome c
Alternative Name: CYCS, CYC
Host: Mouse
Antibody Type: Monoclonal (Clone 7H8.2C12)
Isotype: Mouse IgG2b
Immunogen: Pigeon cytochrome c
Accession#: N/A
Gene ID: N/A
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 0.5 % BSA and 0.01% Thimerosal and 50% glycerol
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |