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Cytochrome c Antibody
Product Overview
Antibody Target: Cytochrome c
Alternative Name: CYCS, CYC
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
Accession#: NP_036972
Gene ID: 25310
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, bovine, chicken, dog
Application: Western blot, Immunoprecipitation
Application & Usage: Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |