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Apaf-1 Antibody
Product Overview
Antibody Target: Apaf-1
Alternative Name: APAF1, CED4, DKFZp781B1145, KIAA0413
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 1161 of human Apaf-1
Accession#: O14727
Gene ID: 20141188
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, bovine, rabbit, monkey, horse
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |