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Caspase-3 (Active) Antibody
Product Overview
Antibody Target: Caspase-3 (Active)
Alternative Name: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Accession#: P42574
Gene ID: 836
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 500 µl antigen affinity purified rabbit anti-Active Caspase-3 antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot, Immunohistochemistry
Application & Usage: Western blotting (1:50 -1:500 dilutions) and Immunohistochemistry (1:10-1:20 dilutions). However, the optimal concentrations should be determined individually. The antibody preferentially recognizes the p17 fragment of the active caspase-3 in samples from human, mouse, and rat origins
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |