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Caspase-3 Antibody (Clone C33)
Product Overview
Antibody Target: Caspase-3
Alternative Name: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Host: Mouse
Antibody Type: Monoclonal (Clone C33)
Isotype: Mouse IgG1
Immunogen: Recombinant human Caspase-3
Accession#: P42574
Gene ID: 836
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (200 µg/ml) in PBS containing 50% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |