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PARP Antibody
Product Overview
Antibody Target: PARP
Alternative Name: PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to caspase cleavage site in human PARP
Accession#: NP_001609
Gene ID: 142
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-PARP polyclonal antibody in phosphatebuffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human
Application: Western blot
Application & Usage: Western blot analysis (1-4 µg/ml). The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP. However, the optimal concentrations should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |