Active Mouse Caspase-8

Caspase-8, mouse recombinant
A cysteine-aspartic acid protease involved in apoptosis
Product Overview
Alternate Names / Synonyms: Active Mouse Caspase-8, Caspase-8 Enzyme, caspase-8, mouse caspase-8, mouse caspases, active caspase-8, caspase-8 assay positive control
Gene Symbol: CASP8
Accession #: Q3U607
Gene ID: 12370
Source: E. coli
Appearance: Lyophilized powder
Physical Form Description: Lyophilized powder
Molecular Weight: N/A
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 5000 units/mg
Reconstitution Instructions: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Storage Temp.: -70°C
Shipping: gel pack
Background Information: Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active mouse caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.