|
Caspase-3 Proform, mouse recombinant
A cysteine-aspartic acid protease involved in apoptosis
Product Overview
Alternate Names / Synonyms: Mouse Procaspase-3, Procaspase-3, procaspases, caspase-3 proenzyme, caspase-3 proform
Gene Symbol: CASP3
Accession #: Q8BNT4
Gene ID: 12367
Source: E. coli
Appearance: Lyophilized powder
Physical Form Description: Lyophilized powder
Molecular Weight: 32.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: Reconstitute to 0.1 µg/µl in PBS containing 15% glycerol.
Storage Temp.: -70°C
Shipping: gel pack
Background Information: Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or “effector” enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and –10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP “apoptosome”. Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. The recombinant Procaspase-3 (1183P-5) is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8 (1088-25, -100). We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100 µl of Reaction Buffer (1068-20) for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits (K105 and K106).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |