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TNF-alpha, rat recombinant
A potent cytokine exerting cytotoxic effects on a wide range of cells
Product Overview
Alternate Names / Synonyms: human TNF-alpha, Human TNF-a, Tumor Necrosis Factor-alpha
Gene Symbol: TNFA
Accession #: P16599.1
Gene ID: 24835
Source: E. Coli.
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered and lyophilized from 1X PBS
Molecular Weight: 17.0 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 10⁷ units/mg.
Reconstitution Instructions: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: gel pack
Background Information: Tumor Necrosis Factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-α is a 17 kDa protein containing 157 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |