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Description: alamarBlue
Name: alamarBlue
Applications: FN
Format: Reagent
Product Type: Accessory Reagent
Quantity: 100 ml
Product Details
Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
Yes No Not Determined Suggested Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Form Liquid
Preservative Stabilisers None present
Specificity alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
Description alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
For further information and Technical help, please visit www.abdserotec.com/alamarBlue.
This site includes:
Frequently Asked Questions
Example calculations
Product-related references
Test Principle • Growing cells cause a chemical reduction of alamarBlue®.
• Continued growth maintains a reduced environment. (fluorescent, red).
• Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation.
• Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
• Absorbance is monitored at 570nm and 600nm.
Intended Use • The bioassay can be used to establish proliferation or relative cytotoxicity.
• Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
• alamarBlue® is for use between pH6.8 and pH7.4.
Storage This product may be stored for 12 months at room temperature.
Store at 2-8oC to increase the shelf life to 20 months.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Shelf Life 12 months from date of despatch.
Acknowledgements Manufactured for AbD Serotec by Trek Diagnostic System. U.S. patent 5,501,959.
Health And Safety Information (A full Health and Safety assessment is available upon request) The REDOX indicator in alamarBlue® has no current or past indication of carcinogenic capacity.
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alamarBlue® indicator dye (BUF012B) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell |