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产地：oxoid
货号：CM0509B
规格：500G
操作说明：
Oxoid Buffered Peptone Water may be used as a pre-enrichment medium, prior to selective enrichment in the isolation of salmonellae from foods. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
It was noted by Edel and Kampelmacher1 that sublethal injury to salmonellae may occur in many food processes. In a survey involving isolation of salmonellae from meat that had been artificially contaminated with sublethally injured organisms, pre-enrichment in buffered peptone water at 37°C for 18 hours before selection in Brilliant Green Tetrathionate Bile Broth showed superior results compared with a direct selection method. Pietzsch2 found that isolation of salmonellae was much improved by pre-enrichment of egg samples in Buffered Peptone Water at 37°C for 18 hours followed by incubation of 10ml of this sample in 100ml Selenite Cystine Broth (CM0699) or Muller-Kauffmann Tetrathionate Broth (CM0343) at 43°C for 48 hours.
Sadovski3 reported that, in experiments involving isolation of salmonellae from frozen vegetables, the rapid drop in pH when using lactose broth4 as a pre-enrichment medium was detrimental to the recovery of salmonellae. This was due to the enhanced sensitivity to low pH of freeze-injured salmonellae which may contaminate frozen vegetables. Pre-enrichment with Buffered Peptone Water maintained a high pH over a period of 24 hours incubation. Vegetable tissue has a low buffering capacity and the medium overcame this problem.
Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature. |