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500g 可以配制17.9 升培养基
NUTRIENT AGAR
Code: CM0003 (Powder)
Code: CM0004 (Tablets)
A general purpose medium which may be enriched with up to 10% blood or other biological fluid.
Directions
Powder: Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Tablets: Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.
Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.
In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms1.
Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.
For a medium which is richer in nutrients, see Blood Agar Base No.2 CM0271.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel |