Biotin RNA Labeling Mix 10x
产品编号:11685597910
产品名称：Biotin RNA Labeling Mix 10x
供应商：Roche
规格：40 μl (20 reactions)
目录价：1815
库存状态：大量现货
相关信息：
Biotin RNA Labeling Mix
Application
This labeling mix can be used (with SP6, T3, or T7 RNA polymerase) to add a nonradioactive label to RNA during in vitro transcription. The labeled RNA can easily and safely be detected (e.g ., with a fluorescein-conjugated or enzyme-conjugated streptavidin).
Product Description
Brief description
The ready-to-use, 10x conc. mix contains:
•ATP, CTP, and GTP, each at a concentration of 10 mM •UTP, 6.5 mM •biotin-16-UTP, 3.5 mMLabeling Efficiency
A standard labeling reaction with 1 µg linear template DNA and an RNA polymerase produces approx. 10 µg of full-length, biotin-labeled RNA. In a spot test, a combination of anti-biotin-AP and the chemiluminescence substrate CSPD can detect as little as 0.3 pg of the biotin-labeled RNA.
Background Information
The biotin-labeled RNA can be used as a hybridization probe for:
•Northern blots •Southern blots •Plaque or colony lifts •RNase protection experiments •In situ hybridization •Microarray hybridization
Contents
Solution, 10x conc., pH 7.5
保存条件： -20-25度