Anti-digoxigenin AP-conjungate

Anti-digoxigenin AP-conjungate|Anti-Digoxigenin-AP, Fab fragments from sheep

产品编号：11093274910     

产品名称：Anti-digoxigenin AP-conjungate|Anti-Digoxigenin-AP, Fab fragments from sheep   

供应商：Roche

规格:150 U (200 μl)

目录价： 2875

库存状态：大量现货

种属来源：地高辛ap-conjungate

相关信息：

Anti-Digoxigenin-AP, Fab fragments from sheep

Application

For the detection of digoxigenin-labeled compounds.

•Dot Blot

•ELISA

•Immunohisto/cytochemistry

•In situ hybridization

•Northern blot

•Southern blot

•Western blot

Product Description

Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Working concentration:

Dot blot: 150 mU/ml

ELISA: 150 - 300 mU/ml

Immunohisto/cytochemistry: 250 - 500 mU/ml

In situ hybridization: 1.5 - 7.5 U/ml

Southern blot: 150 mU/ml

Western blot: 250 - 500 mU/ml 

Background Information

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer. 

Contents

Solution, stabilized