PRESCISSION PROTEASE
产品编号：27-0843-01
产品名称：PRESCISSION PROTEASE
供应商：Pharmacia
规格：500 units
目录价: 4233
库存状态：现货
相关信息：
PreScission Protease
Complete Packsize: 500 units
Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors
Unit Definition: One unit will cleave > 90% of 100 μg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h.
Vector: pGEX-6P-1, pGEX-6P-2, pGEX-6P-3
Molecular Weight [Mr]: 46000
Inhibitor: 100 mM ZnCl2 (< 50% inhibition); 100 μM chymostatin; 4 mM Pefabloc
Elution Buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Cleavage Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0
Supplied As: 2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol.