Pronase from Streptomyces gris
产品编号: 10165921001
产品名称: Pronase from Streptomyces gris
供应商: Roche
规格: 1 g (non-sterile)
目录价: 1028
库存状态: 现货
相关信息:
Pronase
from Streptomyces griseus
For life science research only. Not for use in diagnostic procedures.
Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids.
Contents
Lyophilizate, nonsterile
Total degradation of proteins during the isolation of DNA and RNA; (it is not necessary to let pronase self-digest prior to use). Total hydrolysis of proteins in research and for technical purposes. Tissue dissociation in conjunction with collagenase and trypsin. Production of glycopeptides from purified glycoproteins.
Inhibitors: Complete Protease Inhibitor Cocktail Tablets
Optimum pH: 6.0 - 7.5, depending on type of protease activity
Specific activity: approximately 7,000 U/g lyophilizate at +40°C with casein as the substrate, pH 7.5. One unit is the enzyme activity that liberates folin-positive amino acids and peptides, corresponding to 1 µmol Tyr in 1 minute under assay conditions.
Specificity: Mixture of several nonspecific endo- and exoproteases that generally digest proteins down to single amino acids. Resolves carboxylic acids and alcohols.
Working concentration: 0.5 - 2 mg/ml
Quality
Contaminants: ≤0.002 U DNase/RNase per mg lyophilizate
保存条件: 2-8度