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原装现货|Collagenase D|胶原酶D

发布人:浩然生物 浏览 14594次【字号 】 发布时间:2012年01月30日 打印本页

货号 产品名称 厂家 规格 单价 详细 订购
11088858001 Collagenase D, 100mg Roche 100 mg 845 详细查看 订购
11088882001 Collagenase D, 2.5g, non-steri Roche 2.5 g 11215 详细查看 订购
11088866001 Collagenase D, 500mg Roche 500 mg 2404 详细查看 订购
Application
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures.
Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.
Product Description
EC 3.4.24.3
Enzyme activity:
Collagenase activity: >0.15 U/mg (according to Wünsch) (25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase D has a normal to high collagenase activity and very low tryptic activity (usually <0.2 units/mg, BAEE as substrate).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.
Working concentration: 0.5 - 2.5 mg/ml
Contents
Lyophilizate, non-sterile


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