Key Applications:
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Flow Cytometry
Components:
Equilibration Buffer 90416 3.0 mL -15°C to -25°C
Reaction Buffer 90417 2.0 mL -15°C to -25°C
TdT Enzyme 90418 0.64 mL -15°C to -25°C
Stop/Wash Buffer 90419 20 mL -15°C to -25°C
Blocking Solution 90425 2.6 mL -15°C to -25°C
Anti-Digoxigenin-Fluorescein* 90426 2.1 mL 2°C to 8°C
Plastic Coverslips 90421 100 ea. Room Temp.
*affinity purified sheep polyclonal antibody
Product Overview:
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy.
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.