RevertAid First Strand cDNA Synth 逆转录(RT)试剂

货号	产品名称	厂家	规格	单价	详细	订购
K1622	RevertAid First Strand cDNA Synthesis Kit	MBI	100 react	1600	详细查看	订购

英文名:RevertAid™ First Strand cDNA Synthesis Kit
中文名: 逆转录(RT)试剂盒
RevertAid™ M-MuLV Reverse Transcriptase (200 u*/μl):120 μl
RiboLock™ RNase Inhibitor (20 u**/μl):120 μl
5X Reaction Buffer
250 mM Tris-HCl (pH 8.3), 250 mM KCl, 20 mM MgCl2, 50 mM DTT:500 μl
10mM dNTP Mix:250 μl
Oligo(dT)18 Primer100 μM, 0.5 μg/μl (15 A260 u/ml):120 μl
Random Hexamer Primer100 μM, 0.2 μg/μl (6 A260 u/ml) : 120 μl
Forward GAPDH Primer, 10 μM 5’ – CAAGGTCATCCATGACAACTTTG – 3’: 20 μl
Reverse GAPDH Primer, 10 μM 5’ – GTCCACCACCCTGTTGCTGTAG - 3’:20 μl
Control GAPDH RNA1.3 kb 3’-poly(A) tailed RNA transcript, 0.05 μg/μl:20 μl
Water, nuclease-free:2x1.25 ml