中文名：胰朊酶抑制剂, 胰蛋白酶抑制剂
英文名：Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI
分子量：相对分子质量约 20100
CAS：9035-81-8
性状：无盐冷冻干粉或粗制粉末。对湿敏感。可溶于水和稀缓冲液。酶反应：胰蛋白酶抑制剂＋胰蛋白酶(过量)→[抑制剂•胰蛋白酶]＋胰蛋白酶(剩佘)、 苯甲酰精氨酸乙酯剩余胰蛋白酶苯甲酰精氨酸＋乙醇。除胰蛋白酶外，还能抑制凝血因子Xa、血纤维蛋白溶酶、血浆激肽释放酶。不抑制金属蛋白酶、巯基蛋白酶、酸性蛋白酶、组织激肽释放酶。等电点pI 4.5。最大吸收波长 280nm。能被强酸、强碱变性，在40℃以上可逆变性。在pH 1.0～12.0稀 的缓冲液中稳定。
用途：用亲和色谱法精制胰蛋白酶。血纤维蛋白/血纤维蛋白原无 降解鉴定试验。
Sigma相关产品信息Sigma T9003 Trypsin inhibitor from Glycine max (soybean)
Type I-S, lyophilized powder
Synonym: SBTI
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL number: MFCD00082103
Description
Analysis Note Protein determined by biuret.
One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
Other Notes Extremely stable: a 1% sterile filtered solution maintained activity for over 3 years when kept refrigerated.
View more information on Trypsin Inhibitor.
Preparation Note Chromatographically prepared
Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.
Unit Definition One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
One chymotrypsin unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Biochem/physiol Actions Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yi