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DNeasy Plant Mini Kit

发布人:浩然生物 浏览 2312次【字号 】 发布时间:2017年02月06日 打印本页

DNeasy Plant Mini Kit (50)

产品编号:69104

产品名称:DNeasy Plant Mini Kit (50)

供应商:Qiagen

规格:50T

目录价:2690

库存状态:现货

相关信息:

DNeasy Plant Mini Kit 

植物细胞,组织或真菌细胞样品分离总DNA,最高产量可达 30 µg

纯DNA,无污染物和酶抑制剂

快速纯化得到即用型DNA

无需有机抽提,无需乙醇沉淀

Format: Mini spin columns 

with 2 ml collection tubes 

Sample source: Plant cells and tissues, fungi 

Sample size: Up to 100 mg wet weight 

Preparation time: <1 hour 

Typical yield:* 3&ndash;30 &micro;g 

Elution volume: 50&ndash;400 &micro;l 

Procedure

Samples are first mechanically disrupted and then chemically lysed. RNA is removed by RNase digestion during lysis. Cell debris is removed and samples are filtered and homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted, precipitating proteins and polysaccharides, and the lysate is loaded onto the DNeasy Plant spin column. During a brief spin, DNA selectively binds to the silica-gel membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use (see flowchart "DNeasy Plant and DNeasy 96 Plant Procedures"). 

Downstream applications

The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. DNeasy purified DNA is sized up to 40 kb (see figure "Pure DNA (20&ndash;25 kb) for Restriction Analysis"), and is suitable for downstream applications such as: 

PCR (see figure "PCR Analysis of DNA from Different Plant Species") 



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